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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up documented but specific nucleotide sequence facts isn't still available.
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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide ordinarily consists of the epitope identified by the antibody.
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This information outlines more doable optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This guidebook outlines additional doable optimization of mobile and immunoassay parameters to make certain the best possible outcomes are...
Antibodies certain to the blocking peptide not bind on the epitope about the goal protein. By evaluating the staining from the blocked antibody compared to the antibody by yourself, one can see which staining is unique.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins exhibit distinct expression and degradation styles which add on the temporal coordination of every mitotic occasion. Cyclin E forms a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S section boundary which is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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